A qualitative study on job satisfaction of new medical employees at public hospitals / 中华医院管理杂志

Objective:To understand the real feelings of new medical employees in public hospitals and analyze the influencing factors of their job satisfaction.Methods:From September to December 2019, we adopted the phenomenological research method to conduct semi-structured interviews with 27 new clinical medical staff from three tertiary general hospitals in Beijing for the purpose of understanding their working experience. The 7-step analysis method of Colaizzi phenomenology was used to collate and analyze the data.Results:The five main factors that affect the job satisfaction of new medical staff include job background, job content, expectation and support, interpersonal interaction and hospital culture. Nineteen respondents said they worked long hours and felt overwhelmed. Twenty-five respondents reported that good interpersonal interaction helped improve their work experience.Conclusions:Their satisfaction can be improved by bettering the working environment, implementing person-post value matching, providing emotional support, improving the compensation incentive mechanism, focusing on career development and strengthening humanized management.

Status and dynamic development study of health human resources at a tertiary general hospital in Beijing / 中华医院管理杂志

Objective To analyze challenges existing in the human resources allocation at a tertiary hospital in Beijing and explore tactics for improving health manpower quality and distribution. Methods This study adopted retrospective and descriptive research methods to analyze the basic composition of hospital human resources, namely the numbers, age, educational background and academic titles. It also made descriptive and deductive analysis on the dynamic development of hospital human resources from 2012 to 2017. Results The proportion of hospital staff is balanced in general, yet with too many off-staffs; the proportion of various staff types is irrational. The overall age structure, makeup of education backgrounds to be improved; Lack of high-level talents; Lack of emphasis on career development planning for management and nursing staff. Conclusions The development of health human resources is evolving from quantity growth to that of staff competence improvement and staff makeup optimization. The hospital should explore and innovate approaches in staffing reform, draft manpower development plan scientifically, explore innovation means to make human scientific development planning, optimize the structure of human resources, innovate the talent evaluation mechanism and give full play to the incentive role of talent evaluation, guide professional training programs for health professionals, improve the efficiency of health human resources and work for balanced and coordinated development.

ERK signaling pathway mediated epithelial-mesenchymal transition induced by SiO₂ in human bronchial epithelial cells / 中南大学学报(医学版)

OBJECTIVE@#To determine the role of extracellular signal regulated kinase (ERK) signaling pathway in SiO₂ induced epithelial-mesenchymal transition (EMT) in human bronchial epithelial cells (HBEC) in vitro.@*METHODS@#HBEC were treated with SiO₂ (0-300 μg/mL) for 72 h or pretreated with U0126 (0-30 μmol/L) for 1 h and then treated with 200 μg/mL SiO₂ for 72 h. Western blot was used to detect the protein expression of E-cadherin and α-smooth muscle actin (α-SMA). The activity of ERK was examined by mitogen-activated protein kinase (MAPK) activity assay kit in HBEC exposing to SiO₂ (200 μg/mL) for 0-8 h.@*RESULTS@#The expression of E-cadherin decreased gradually in SiO₂ -stimulated HBEC, and the effect was most significant at 300 μg/mL (P<0.01). The expression of α-SMA increased and the effect was most evident at 200 μg/mL (P<0.01). With SiO₂ treatment, the activity of ERK was upregulated significantly. The phosphorylation of ERK increased at 30 min and decreased after 1 h. U0126 significantly inhibited SiO₂ -induced expression changes in E-cadherin and α-SMA. At 30 μmol/L, the effect was most evident(P<0.01).@*CONCLUSION@#ERK signaling pathway mediated EMT induced by SiO₂ in HBEC.

Effect of Rho signaling pathway on SiO2 induced α-SMA expression in human bronchial epithelial cells / 中南大学学报(医学版)

Objective To investigate the role of Rho in SiO2 induced α-SMA expression in human bronchial epithelial cells (HBECs). Methods HBECs were cultured and stimulated with SiO2. Immunocytochemistry and Western blot were used to detect the expression of α-SMA. The activity of Rho was determined by GST pull down assay. In the prevention experiment,SiO2-stimulated HBECs were incubated with Rho inhibitor Y27632,and the expression of α-SMA was examined by Western blot. Results With SiO2 (0-300 μg/mL) treatment,the expression of α-SMA increased gradually,and 200 μg/mL of SiO2 led to the highest expression of α-SMA which was (5.09±1.98) times of the expression of α-SMA in the control group(P＜0.01). HBECs treated with SiO2 (200 μg/mL) for indicated time (1,2,6,12,and 24 h)showed an obvious increase of Rho activity(P＜0.01). Y27632 inhibited SiO2-induced α-SMA expression significantly,and the inhibition rate of 20 and 30 μmol/L Y27632 was 68% and 75%,respectively (P＜0.01).Conclusion Rho signaling pathway may mediate SiO2 induced α-SMA expression in HBECs.